Isolation of plasma and nuclear membranes of thymocytes. II. Biochemical composition
نویسندگان
چکیده
منابع مشابه
Isolation of plasma and nuclear membranes of thymocytes. II. Biochemical composition
Thymocyte plasma and nuclear membranes obtained by the procedure described in the accompanying paper were analyzed for their biochemical composition. Plasma membranes were very rich in phospholipid, cholesterol, sialic aicd; they did not contain nucleic acids. In comparison, nuclear membranes had a lower phospholipid to protein ratio and contained much less sialic acid and cholesterol. 50% of t...
متن کاملIsolation of plasma and nuclear membranes of thymocytes. I. Enzymatic composition and ultrastructure
The purpose of this work was to isolate thymocyte plasma membranes at high yield and purity to study specific surface molecules in their structural context. A procedure was developed in which 92-95% of the cells were disrupted by homogenization in a dense viscous medium, while nuclei remained intact. Differential centrifugation of the homogenate was avoided; instead, only a brief (2 h) centrifu...
متن کاملNUCLEAR MEMBRANES FROM MAMMALIAN LIVER II. Lipid Composition
The qualitative and quantitative lipid composition of nuclei and nuclear membranes from pig and rat liver were determined . These determinations were compared with the corresponding data obtained for microsomes from the same material after similar treatments . The results indicate that, at least, by far the major part of the nuclear lipids is located in the membranes of the nuclear envelope . T...
متن کاملIsolation of Rat Liver Plasma Membranes
Nucleotide pyrophosphatase and phosphodiesterase I of rat liver have been found to be localized primarily in cell particulates highly enriched with respect to the most commonly accepted plasma membrane marker, 5'-nucleotidase, and therefore should themselves be assigned a plasma membrane localization. The observation that plasma membranes sediment in isotonic sucrose with both nuclear and micro...
متن کاملThe purification and biochemical characterization of bovine liver nuclear membranes.
Bovine liver nuclear membranes were purified by means of a high salt (0.5 M MgCl?) discontinuous sucrose gradient. The purified nuclear membranes were isolated at a density of p = 1.05 to 1.23 and contained 62% of the protein recovered from the gradient. The presence of nuclear pore complexes identified the fraction as nuclear membrane. Twenty-six per cent of the protein was recovered at a dens...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1978
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.77.1.232